The chicken beta/epsilon-globin enhancer directs autonomously regulated, high-level expression of the chicken epsilon-globin gene in transgenic mice.
نویسندگان
چکیده
منابع مشابه
Chicken globin gene number.
Using complementary DNA prepared from adult chicken globin messenger RNA, we show the existence of 2-3 non-cross-hybridising globin sequences in the chicken genome, each of which is present in only one copy per haploid genome. This was done by solution hybridization to total DNA under conditions of cDNA excess. The data agreses with results of RNA-driven hybridisation of globin complementary DN...
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We reported previously that DNA bend sites appear in the human /3-globin locus at an average distance of 680 bp. The relative locations of the sites were conserved among the five active /3-like globin genes and one pseudogene. Here, we mapped the sites in the /3-like globin genes from various species and examined their conservation. The locations of the bend sites in the bovine, rabbit and chic...
متن کاملCTCF-dependent enhancer blockers at the upstream region of the chicken alpha-globin gene domain.
The eukaryotic genome is partitioned into chromatin domains containing coding and intergenic regions. Insulators have been suggested to play a role in establishing and maintaining chromatin domains. Here we describe the identification and characterization of two separable enhancer blocking elements located in the 5' flanking region of the chicken alpha-globin domain, 11-16 kb upstream of the em...
متن کاملRegulation of globin genes expression: New findings made with the chicken domain of α globin genes
_________________________________________________________________________________________________ *Correspondence: Razin, Sergey, Ph.D, Institute of Gene Biology RAS, Vavilov Str. 34/5, 117334 Moscow, Russia e-mail: [email protected], [email protected],[email protected]
متن کاملAnalysis of the Human {-Globin Gene Promoter in Transgenic Mice
{-Globin is the embryonic form of the O( chain of hemoglobin. Transgenic mice generated with {-globin constructs containing the {-globin gene, 557 bp of 5 flanking sequence, and 2-kb of 3 flanking sequence linked to the @globin locus control region hypersensitive site 2 (HS2) expressed human {-globin only in embryonic yolk sac erythroid tissue, and not in definitive erythroid tissue in the feta...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1994
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.91.15.7252